Application

Western blot: 1:1,000 using ECL on rat brain lysate.

Immunohistochemistry: 1:1,000.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Anti-NMDAR1 Splice Variant C1 Antibody is an antibody against NMDAR1 Splice Variant C1 for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide corresponding to the C-terminus of the rat NMDAR1 C1 splice variant.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 µL of PBS. Contains no preservatives.

Specificity

Specific for the NMDAR1 C1 splice variant. AB5046P recognizes the C1 splice insert present in NR1 variants, 1a, 1b, 3a and 3b. {Gorter et al, (1997), "The Role of Alternative Splicing of the NMDAR1 Receptor Subunit in Synaptic Plasticity" Chapter 4, pg 99, in The Ionotropic Glutamate Receptors , Monaghan, D.T. and Weinhold, R.J. (eds), Humana Press, New Jersey) By Western blot the antibody recognizes a protein with molecular weight of 100 kDa.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.